Quadriwave lateral shearing interferometry for quantitative phase microscopy of living cells.
Abstrak
Phase imaging with a high-resolution wavefront sensor is considered. This is based on a quadriwave lateral shearing interferometer mounted on a non-modified transmission white-light microscope. The measurement technology is explained both in the scope of wave optics and geometrical optics in order to discuss its implementation on a conventional microscope. In particular we consider the effect of a non spatially coherent source on the phase-image signal-to-noise ratio. Precise measurements of the phase-shift introduced by microscopic beads or giant unilamellar vesicles validate the principle and show the accuracy of the methods. Diffraction limited images of living COS-7 cells are then presented, with a particular focus on the membrane and organelle dynamics.
Topik & Kata Kunci
Penulis (4)
P. Bon
Guillaume Maucort
B. Wattellier
S. Monneret
Akses Cepat
- Tahun Terbit
- 2009
- Bahasa
- en
- Total Sitasi
- 471×
- Sumber Database
- Semantic Scholar
- DOI
- 10.1364/OE.17.013080
- Akses
- Open Access ✓