Semantic Scholar Open Access 2019 189 sitasi

Cartography of opportunistic pathogens and antibiotic resistance genes in a tertiary hospital environment

Kern Rei Chng Chenhao Li D. Bertrand A. Ng Junmei Samantha Kwah +106 lainnya

Lihat Sumber DOI

Abstrak

Although disinfection is key to infection control, the colonization patterns and resistomes of hospital-environment microbes remain underexplored. We report the first extensive genomic characterization of microbiomes, pathogens and antibiotic resistance cassettes in a tertiary-care hospital, from repeated sampling (up to 1.5 years apart) of 179 sites associated with 45 beds. Deep shotgun metagenomics unveiled distinct ecological niches of microbes and antibiotic resistance genes characterized by biofilm-forming and human-microbiome-influenced environments with corresponding patterns of spatiotemporal divergence. Quasi-metagenomics with nanopore sequencing provided thousands of high-contiguity genomes, phage and plasmid sequences (>60% novel), enabling characterization of resistome and mobilome diversity and dynamic architectures in hospital environments. Phylogenetics identified multidrug-resistant strains as being widely distributed and stably colonizing across sites. Comparisons with clinical isolates indicated that such microbes can persist in hospitals for extended periods (>8 years), to opportunistically infect patients. These findings highlight the importance of characterizing antibiotic resistance reservoirs in hospitals and establish the feasibility of systematic surveys to target resources for preventing infections. Spatiotemporal characterization of microbial diversity and antibiotic resistance in a tertiary-care hospital reveals broad distribution and persistence of antibiotic-resistant organisms that could cause opportunistic infections in a healthcare setting.

Topik & Kata Kunci

Biology Medicine

Penulis (111)

Kern Rei Chng

Chenhao Li

D. Bertrand

A. Ng

Junmei Samantha Kwah

Hwee Meng Low

C. Tong

Maanasa Natrajan

M. Zhang

Licheng Xu

K. Ko

E. Ho

T. Av-Shalom

J. Teo

C. Khor

David Daniela Ebrahim Sofia Chandrima Daniel J. Kern Rei Danko Bezdan Afshinnekoo Ahsanuddin Bhattacharya B

D. Danko

D. Bezdan

Ebrahim Afshinnekoo

Sofia Ahsanuddin

Chandrima Bhattacharya

D. Butler

Kern Rei Chng

F. De Filippis

J. Hecht

A. Kahles

Mikhail Karasikov

N. Kyrpides

M. Leung

D. Meleshko

Harun Mustafa

B. Mutai

R. Neches

A. Ng

M. Nieto-Caballero

O. Nikolayeva

T. Nikolayeva

E. Png

Jorge L. Sanchez

H. Shaaban

Maria A. Sierra

X. Tong

B. Young

Josue Alicea

Malay Bhattacharyya

R. Blekhman

E. Castro-Nallar

Ana M. Cañas

A. Chatziefthimiou

Robert W. Crawford

You-Peng Deng

C. Desnues

E. Dias-Neto

Daisy Donnellan

M. Dybwad

E. Elhaik

D. Ercolini

A. Frolova

A. Graf

D. C. Green

I. Hajirasouliha

Mark Hernandez

G. Iraola

Soojin Jang

Angela Jones

F. Kelly

Kaymisha Knights

Paweł P. Łabaj

P. Lee

L. Shawn

P. Ljungdahl

Abigail Lyons

G. Mason-Buck

K. McGrath

E. Mongodin

M. Moraes

Niranjan Nagarajan

H. Noushmehr

Manuela Oliveira

S. Ossowski

O. Osuolale

Orhan Özcan

David Páez-Espino

N. Rascovan

Hugues Richard

G. Rätsch

L. Schriml

T. Semmler

O. U. Sezerman

Leming Shi

L. Song

Haruo Suzuki

D. Court

Dominique Thomas

S. Tighe

K. Udekwu

J. Ugalde

Brandon Valentine

D. Vassilev

Elena M. Vayndorf

T. Velavan

M. Zambrano

Jifeng Zhu

Sibo Zhu

Christopher E. Mason

S. Chen

Christopher E. Mason

O. Ng

K. Marimuthu

B. Ang

Niranjan Nagarajan

Format Sitasi

APA MLA BibTeX

Chng, K.R., Li, C., Bertrand, D., Ng, A., Kwah, J.S., Low, H.M. et al. (2019). Cartography of opportunistic pathogens and antibiotic resistance genes in a tertiary hospital environment. https://doi.org/10.1038/s41591-020-0894-4

@article{chng2019, author = {Kern Rei Chng and Chenhao Li and D. Bertrand and A. Ng and Junmei Samantha Kwah and Hwee Meng Low and C. Tong and Maanasa Natrajan and M. Zhang and Licheng Xu and K. Ko and E. Ho and T. Av-Shalom and J. Teo and C. Khor and David Daniela Ebrahim Sofia Chandrima Daniel J. Kern Rei Danko Bezdan Afshinnekoo Ahsanuddin Bhattacharya B and D. Danko and D. Bezdan and Ebrahim Afshinnekoo and Sofia Ahsanuddin and Chandrima Bhattacharya and D. Butler and Kern Rei Chng and F. De Filippis and J. Hecht and A. Kahles and Mikhail Karasikov and N. Kyrpides and M. Leung and D. Meleshko and Harun Mustafa and B. Mutai and R. Neches and A. Ng and M. Nieto-Caballero and O. Nikolayeva and T. Nikolayeva and E. Png and Jorge L. Sanchez and H. Shaaban and Maria A. Sierra and X. Tong and B. Young and Josue Alicea and Malay Bhattacharyya and R. Blekhman and E. Castro-Nallar and Ana M. Cañas and A. Chatziefthimiou and Robert W. Crawford and You-Peng Deng and C. Desnues and E. Dias-Neto and Daisy Donnellan and M. Dybwad and E. Elhaik and D. Ercolini and A. Frolova and A. Graf and D. C. Green and I. Hajirasouliha and Mark Hernandez and G. Iraola and Soojin Jang and Angela Jones and F. Kelly and Kaymisha Knights and Paweł P. Łabaj and P. Lee and L. Shawn and P. Ljungdahl and Abigail Lyons and G. Mason-Buck and K. McGrath and E. Mongodin and M. Moraes and Niranjan Nagarajan and H. Noushmehr and Manuela Oliveira and S. Ossowski and O. Osuolale and Orhan Özcan and David Páez-Espino and N. Rascovan and Hugues Richard and G. Rätsch and L. Schriml and T. Semmler and O. U. Sezerman and Leming Shi and L. Song and Haruo Suzuki and D. Court and Dominique Thomas and S. Tighe and K. Udekwu and J. Ugalde and Brandon Valentine and D. Vassilev and Elena M. Vayndorf and T. Velavan and M. Zambrano and Jifeng Zhu and Sibo Zhu and Christopher E. Mason and S. Chen and Christopher E. Mason and O. Ng and K. Marimuthu and B. Ang and Niranjan Nagarajan}, title = {Cartography of opportunistic pathogens and antibiotic resistance genes in a tertiary hospital environment}, year = {2019}, journal = {Semantic Scholar}, doi = {10.1038/s41591-020-0894-4}, url = {https://www.semanticscholar.org/paper/749dcb3f9dc74da18882a3d51a7e6cdd871468b8}, }

Akses Cepat

Lihat di Sumber doi.org/10.1038/s41591-020-0894-4

Informasi Jurnal

Tahun Terbit: 2019
Bahasa: en
Total Sitasi: 189×
Sumber Database: Semantic Scholar
DOI: 10.1038/s41591-020-0894-4
Akses: Open Access ✓