Semantic Scholar Open Access 2011 1572 sitasi

Improving RNA-Seq expression estimates by correcting for fragment bias

Adam Roberts C. Trapnell Julie Donaghey J. Rinn L. Pachter

Lihat Sumber DOI

Abstrak

The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based approach. We find improvements in expression estimates as measured by correlation with independently performed qRT-PCR and show that correction of bias leads to improved replicability of results across libraries and sequencing technologies.

Topik & Kata Kunci

Biology Medicine

Penulis (5)

Adam Roberts

C. Trapnell

Julie Donaghey

J. Rinn

L. Pachter

Format Sitasi

APA MLA BibTeX

Roberts, A., Trapnell, C., Donaghey, J., Rinn, J., Pachter, L. (2011). Improving RNA-Seq expression estimates by correcting for fragment bias. https://doi.org/10.1186/gb-2011-12-3-r22

Akses Cepat

Lihat di Sumber doi.org/10.1186/gb-2011-12-3-r22

Informasi Jurnal

Tahun Terbit: 2011
Bahasa: en
Total Sitasi: 1572×
Sumber Database: Semantic Scholar
DOI: 10.1186/gb-2011-12-3-r22
Akses: Open Access ✓