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Semantic Scholar Open Access 2019 1456 sitasi

Conserved cell types with divergent features in human versus mouse cortex

R. Hodge Trygve E Bakken Jeremy A. Miller Kimberly A. Smith Eliza R. Barkan +59 lainnya
Lihat Sumber DOI

Abstrak

Elucidating the cellular architecture of the human cerebral cortex is central to understanding our cognitive abilities and susceptibility to disease. Here we used single-nucleus RNA-sequencing analysis to perform a comprehensive study of cell types in the middle temporal gyrus of human cortex. We identified a highly diverse set of excitatory and inhibitory neuron types that are mostly sparse, with excitatory types being less layer-restricted than expected. Comparison to similar mouse cortex single-cell RNA-sequencing datasets revealed a surprisingly well-conserved cellular architecture that enables matching of homologous types and predictions of properties of human cell types. Despite this general conservation, we also found extensive differences between homologous human and mouse cell types, including marked alterations in proportions, laminar distributions, gene expression and morphology. These species-specific features emphasize the importance of directly studying human brain. RNA-sequencing analysis of cells in the human cortex enabled identification of diverse cell types, revealing well-conserved architecture and homologous cell types as well as extensive differences when compared with datasets covering the analogous region of the mouse brain.

Topik & Kata Kunci

Medicine Biology

Penulis (64)

R

R. Hodge

T

Trygve E Bakken

J

Jeremy A. Miller

K

Kimberly A. Smith

E

Eliza R. Barkan

L

Lucas T. Graybuck

J

J. Close

B

Brian R. Long

N

Nelson J. Johansen

O

Osnat Penn

Z

Zizhen Yao

J

J. Eggermont

T

T. Höllt

B

Boaz P. Levi

S

Soraya I Shehata

B

B. Aevermann

A

Allison Beller

D

Darren Bertagnolli

K

Krissy Brouner

T

Tamara Casper

C

C. Cobbs

R

R. Dalley

N

N. Dee

S

S. Ding

R

R. Ellenbogen

O

Olivia Fong

E

E. Garren

J

Jeff Goldy

R

R. Gwinn

D

Daniel Hirschstein

C

C. Keene

M

Mohamed Keshk

A

A. Ko

K

K. Lathia

A

A. Mahfouz

Z

Zoe Maltzer

M

M. McGraw

T

T. Nguyen

J

J. Nyhus

J

J. Ojemann

A

Aaron Oldre

S

Sheana E. Parry

S

Shannon Reynolds

C

Christine Rimorin

N

N. Shapovalova

S

Saroja Somasundaram

A

A. Szafer

E

Elliot R. Thomsen

M

Michael Tieu

G

G. Quon

R

R. Scheuermann

R

R. Yuste

S

S. Sunkin

B

B. Lelieveldt

D

David Feng

L

L. Ng

A

Amy Bernard

M

M. Hawrylycz

J

John W. Phillips

B

Bosiljka Tasic

H

Hongkui Zeng

A

Allan R. Jones

C

C. Koch

E

E. Lein

Format Sitasi

APA MLA BibTeX
Hodge, R., Bakken, T.E., Miller, J.A., Smith, K.A., Barkan, E.R., Graybuck, L.T. et al. (2019). Conserved cell types with divergent features in human versus mouse cortex. https://doi.org/10.1038/s41586-019-1506-7

Akses Cepat

Lihat di Sumber doi.org/10.1038/s41586-019-1506-7
Informasi Jurnal
Tahun Terbit
2019
Bahasa
en
Total Sitasi
1456×
Sumber Database
Semantic Scholar
DOI
10.1038/s41586-019-1506-7
Akses
Open Access ✓