Spectrophotometric Determination of Flavonoids Scrophularia nodosa L., widespread in the Shahbuz Region of the Nakhchivan Autonomous Republic

Shahbuz region, considered the “flora museum” of the Nakhchivan Autonomous Republic, occupies a special place in the Autonomous Republic due to the richness of its biodiversity, especially the diversity of flora species. In fact, this richness is due to the many species contained in the flora of the region, including wild vegetables, honey and nectar plants, medicinal plants, as well as essences, alkaloids, saponins, flavonoids, glycosides, grafting agents, etc. Medicinal plants are of particular importance in this wealth. Some plant raw materials contain bioactive compounds with a wide range of effects, and the study and application of various substances in these mixtures, especially flavonoids, has always been the focus of scientific attention. Particularly, the high biological activity of flavonoids, their broader pharmacological effects and abundance in the plant kingdom should be considered as the main reasons for conducting research. A number of flavonoids have antibacterial effects. Thanks to their antioxidant properties, flavonoids protect the body from harmful environmental influences. In particular, the use of these substances in medicine and cosmetics, the pharmaceutical and food industries leads to a further increase in interest in them. Considering that the species Scrophularia nodosa L., widespread in the Nakhchivan Autonomous Region, including the Shahbuz region, is rich in flavonoids, qualitative analyzes were carried out to prove the presence of flavonoids in Scrophularia nodosa, and the amount of flavonoids was determined by the spectrophotometric method. As a result of laboratory studies, the total amount of flavonoids, determined by spectrophotometric method in the form of Scrophularia nodosa L., was 5,58%.