Semantic Scholar Open Access 2022 5 sitasi

FOXO1 Is Present in Stomach Epithelium and Determines Gastric Cell Distribution

Wendy M. McKimpson Taiyi Kuo T. Kitamoto Sei Higuchi J. Mills +2 lainnya

Lihat Sumber DOI

Abstrak

Background and Aims Stomach cells can be converted to insulin-producing cells by Neurog3, MafA, and Pdx1 overexpression. Enteroendocrine cells can be similarly made to produce insulin by the deletion of FOXO1. Characteristics and functional properties of FOXO1-expressing stomach cells are not known. Methods Using mice bearing a FOXO1-GFP knock-in allele and primary cell cultures, we examined the identity of FOXO1-expressing stomach cells and analyzed their features through loss-of-function studies with red-to-green fluorescent reporters. Results FOXO1 localizes to a subset of Neurog3 and parietal cells. FOXO1 deletion ex vivo or in vivo using Neurog3-cre or Atp4b-cre increased numbers of parietal cells, generated insulin- and C-peptide-immunoreactive cells, and raised Neurog3 messenger RNA. Gene expression and ChIP-seq experiments identified the cell cycle regulator cyclin E1 (CCNE1) as a FOXO1 target. Conclusion FOXO1 is expressed in a subset of stomach cells. Its ablation increases parietal cells and yields insulin-immunoreactive cells, consistent with a role in lineage determination.

Topik & Kata Kunci

Medicine

Penulis (7)

Wendy M. McKimpson

Taiyi Kuo

T. Kitamoto

Sei Higuchi

J. Mills

R. Haeusler

D. Accili

Format Sitasi

APA MLA BibTeX

McKimpson, W.M., Kuo, T., Kitamoto, T., Higuchi, S., Mills, J., Haeusler, R. et al. (2022). FOXO1 Is Present in Stomach Epithelium and Determines Gastric Cell Distribution. https://doi.org/10.1016/j.gastha.2022.05.005

Akses Cepat

Lihat di Sumber doi.org/10.1016/j.gastha.2022.05.005

Informasi Jurnal

Tahun Terbit: 2022
Bahasa: en
Total Sitasi: 5×
Sumber Database: Semantic Scholar
DOI: 10.1016/j.gastha.2022.05.005
Akses: Open Access ✓