Semantic Scholar Open Access 2018 407 sitasi

Software tools for automated transmission electron microscopy

Martin Schorb I. Haberbosch W. Hagen Y. Schwab D. Mastronarde

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Abstrak

The demand for high-throughput data collection in electron microscopy is increasing for applications in structural and cellular biology. Here we present a combination of software tools that enable automated acquisition guided by image analysis for a variety of transmission electron microscopy acquisition schemes. SerialEM controls microscopes and detectors and can trigger automated tasks at multiple positions with high flexibility. Py-EM interfaces with SerialEM to enact specimen-specific image-analysis pipelines that enable feedback microscopy. As example applications, we demonstrate dose reduction in cryo-electron microscopy experiments, fully automated acquisition of every cell in a plastic section and automated targeting on serial sections for 3D volume imaging across multiple grids. Py-EM and SerialEM enable automated microscope control for high-throughput data acquisition in diverse transmission electron microscopy imaging experiments.

Topik & Kata Kunci

Medicine Biology Computer Science

Penulis (5)

Martin Schorb

I. Haberbosch

W. Hagen

Y. Schwab

D. Mastronarde

Format Sitasi

APA MLA BibTeX

Schorb, M., Haberbosch, I., Hagen, W., Schwab, Y., Mastronarde, D. (2018). Software tools for automated transmission electron microscopy. https://doi.org/10.1038/s41592-019-0396-9

Akses Cepat

Lihat di Sumber doi.org/10.1038/s41592-019-0396-9

Informasi Jurnal

Tahun Terbit: 2018
Bahasa: en
Total Sitasi: 407×
Sumber Database: Semantic Scholar
DOI: 10.1038/s41592-019-0396-9
Akses: Open Access ✓