Semantic Scholar Open Access 2022 49 sitasi

The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance

G. La Rosa M. Iaconelli C. Veneri P. Mancini G. Bonanno Ferraro +147 lainnya

Lihat Sumber DOI

Abstrak

The SARS-CoV-2 Omicron variant emerged in South Africa in November 2021, and has later been identified worldwide, raising serious concerns. A real-time RT-PCR assay was designed for the rapid screening of the Omicron variant, targeting characteristic mutations of the spike gene. The assay was used to test 737 sewage samples collected throughout Italy (19/21 Regions) between 11 November and 25 December 2021, with the aim of assessing the spread of the Omicron variant in the country. Positive samples were also tested with a real-time RT-PCR developed by the European Commission, Joint Research Centre (JRC), and through nested RT-PCR followed by Sanger sequencing. Overall, 115 samples tested positive for Omicron SARS-CoV-2 variant. The first occurrence was detected on 7 December, in Veneto, North Italy. Later on, the variant spread extremely fast in three weeks, with prevalence of positive wastewater samples rising from 1.0% (1/104 samples) in the week 5–11 December, to 17.5% (25/143 samples) in the week 12–18, to 65.9% (89/135 samples) in the week 19–25, in line with the increase in cases of infection with the Omicron variant observed during December in Italy. Similarly, the number of Regions/Autonomous Provinces in which the variant was detected increased from one in the first week, to 11 in the second, and to 17 in the last one. The presence of the Omicron variant was confirmed by the JRC real-time RT-PCR in 79.1% (91/115) of the positive samples, and by Sanger sequencing in 66% (64/97) of PCR amplicons. In conclusion, we designed an RT-qPCR assay capable to detect the Omicron variant, which can be successfully used for the purpose of wastewater-based epidemiology. We also described the history of the introduction and diffusion of the Omicron variant in the Italian population and territory, confirming the effectiveness of sewage monitoring as a powerful surveillance tool.

Topik & Kata Kunci

Medicine

Penulis (152)

G. La Rosa

M. Iaconelli

C. Veneri

P. Mancini

G. Bonanno Ferraro

D. Brandtner

L. Lucentini

L. Bonadonna

M. Rossi

M. Grigioni

Achille Alberta Alberto Alberto Alessandra Alessandra Amal Palma Stenico Antonelli Izzotti Schiavuzzi Tosco P

A. Palma

A. Stenico

Alberto Antonelli

A. Izzotti

Alessandra Schiavuzzi

A. Tosco

A. Porta

A. Franzetti

A. Turolla

Angela Costa

A. D'Argenzio

A. Romano

Anna Pariani

A. Carducci

Anna-Maria Prast

A. Agodi

A. Cersini

Antonio Pizzolante

A. Azzellino

B. Bertasi

B. Griglio

C. Ancona

C. Maida

Carmen Montanaro

C. Ottaviano

C. Cocuzza

C. Pignata

Daniele Nasci

D. Cereda

Desdemona Oliva

D. A. Giorgi

E. Grasselli

Elena Mengon

E. Nicosia

E. Carraro

E. Ammoni

E. Ricci

Eric Grange

E. Federici

E. Zuccato

F. Filippetti

F. Zuccon

F. Guarneri

Florida Damasco

F. Palumbo

F. Apollonio

Francesca Borney

F. Ciuti

F. Cutrupi

F. Malpei

F. Pennino

F. Russo

F. Pizzo

F. Triggiano

Franco Rigoli

Gabriella Trani

G. Cecchini

Gianluca Borlone

Giorgia Allaria

G. Bertanza

G. Fusco

Giovanna La Vecchia

G. Alborali

G. Giammanco

Giovanni Santoro

G. Pitter

Giulia Lauretani

Giulia Nani

G. Purpari

G. Aprea

G. Bucciarelli

G. Di Vittorio

G. Lauria

I. Federigi

I. Amoruso

I. Ferrante

L. De Lellis

L. Gentili

Lorella Zago

L. Decastelli

L. Bolognini

Luigi Cossentino

M. Bianchi

M. Antonelli

M. Guercio

M. Verani

Marco Zampini

M. Ferrante

M. Cadonna

M. G. Cerroni

M. Callegari

M. Montagna

M. Scicluna

M. Arizzi

M. Mariuz

M. Losio

M. Palermo

M. Paniccia’

M. Triassi

M. Barchitta

M. Ramazzotti

M. Postinghel

Mauro Cravero

M. Ruffier

Maya Petricciuolo

Michele La Bianca

Michele Colitti

M. Monfrinotti

N. Fontani

N. Ungaro

N. Formenti

Onofrio Mongelli

O. De Giglio

P. Angelini

P. Foladori

Paolo Torlontano

Patrizia Montenegro

P. Laura

Piergiuseppe Calà

Renato Olivares

Renza Berruti

R. Cifarelli

Rosanna Brienza

S. Binda

S. Briscolini

S. Castiglioni

Sara Muzio

S. Manara

S. Bonetta

S. Magi

Silvia Riosa

S. Schiarea

S. De Grazia

Sofia Barigelli

Stefano Rosatto

T. Baldovin

Valeria Capparuccini

V. Primache

Vanessa Groppi

W. Mazzucco

E. Suffredini

Format Sitasi

APA MLA BibTeX

Rosa, G.L., Iaconelli, M., Veneri, C., Mancini, P., Ferraro, G.B., Brandtner, D. et al. (2022). The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance. https://doi.org/10.1016/j.scitotenv.2022.155767

@article{rosa2022, author = {G. La Rosa and M. Iaconelli and C. Veneri and P. Mancini and G. Bonanno Ferraro and D. Brandtner and L. Lucentini and L. Bonadonna and M. Rossi and M. Grigioni and Achille Alberta Alberto Alberto Alessandra Alessandra Amal Palma Stenico Antonelli Izzotti Schiavuzzi Tosco P and A. Palma and A. Stenico and Alberto Antonelli and A. Izzotti and Alessandra Schiavuzzi and A. Tosco and A. Porta and A. Franzetti and A. Turolla and Angela Costa and A. D'Argenzio and A. Romano and Anna Pariani and A. Carducci and Anna-Maria Prast and A. Agodi and A. Cersini and Antonio Pizzolante and A. Azzellino and B. Bertasi and B. Griglio and C. Ancona and C. Maida and Carmen Montanaro and C. Ottaviano and C. Cocuzza and C. Pignata and Daniele Nasci and D. Cereda and Desdemona Oliva and D. A. Giorgi and E. Grasselli and Elena Mengon and E. Nicosia and E. Carraro and E. Ammoni and E. Ricci and Eric Grange and E. Federici and E. Zuccato and F. Filippetti and F. Zuccon and F. Guarneri and Florida Damasco and F. Palumbo and F. Apollonio and Francesca Borney and F. Ciuti and F. Cutrupi and F. Malpei and F. Pennino and F. Russo and F. Pizzo and F. Triggiano and Franco Rigoli and Gabriella Trani and G. Cecchini and Gianluca Borlone and Giorgia Allaria and G. Bertanza and G. Fusco and Giovanna La Vecchia and G. Alborali and G. Giammanco and Giovanni Santoro and G. Pitter and Giulia Lauretani and Giulia Nani and G. Purpari and G. Aprea and G. Bucciarelli and G. Di Vittorio and G. Lauria and I. Federigi and I. Amoruso and I. Ferrante and L. De Lellis and L. Gentili and Lorella Zago and L. Decastelli and L. Bolognini and Luigi Cossentino and M. Bianchi and M. Antonelli and M. Guercio and M. Verani and Marco Zampini and M. Ferrante and M. Cadonna and M. G. Cerroni and M. Callegari and M. Montagna and M. Scicluna and M. Arizzi and M. Mariuz and M. Losio and M. Palermo and M. Paniccia’ and M. Triassi and M. Barchitta and M. Ramazzotti and M. Postinghel and Mauro Cravero and M. Ruffier and Maya Petricciuolo and Michele La Bianca and Michele Colitti and M. Monfrinotti and N. Fontani and N. Ungaro and N. Formenti and Onofrio Mongelli and O. De Giglio and P. Angelini and P. Foladori and Paolo Torlontano and Patrizia Montenegro and P. Laura and Piergiuseppe Calà and Renato Olivares and Renza Berruti and R. Cifarelli and Rosanna Brienza and S. Binda and S. Briscolini and S. Castiglioni and Sara Muzio and S. Manara and S. Bonetta and S. Magi and Silvia Riosa and S. Schiarea and S. De Grazia and Sofia Barigelli and Stefano Rosatto and T. Baldovin and Valeria Capparuccini and V. Primache and Vanessa Groppi and W. Mazzucco and E. Suffredini}, title = {The rapid spread of SARS-COV-2 Omicron variant in Italy reflected early through wastewater surveillance}, year = {2022}, journal = {Semantic Scholar}, doi = {10.1016/j.scitotenv.2022.155767}, url = {https://www.semanticscholar.org/paper/14f4095f7a5659be5518134c9c31ef1efb18dcf0}, }

Akses Cepat

Lihat di Sumber doi.org/10.1016/j.scitotenv.2022.155767

Informasi Jurnal

Tahun Terbit: 2022
Bahasa: en
Total Sitasi: 49×
Sumber Database: Semantic Scholar
DOI: 10.1016/j.scitotenv.2022.155767
Akses: Open Access ✓