DOAJ Open Access 2013

PARTIAL PURIFICATION AND IMMUNE-BIOCHEMICAL CHARACTERIZATION OF DOG SERUM IMMUNOGLOBULIN G

Manoj Kumar Subhasis Batabyal Shyamsundar Kesh Saibal Chattopadhyay

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Abstrak

In the present study Immunoglobulin G was purified from serum of dog by gel filtration chromatography on Sephacryl S-200. SDS- PAGE analysis of purified dog IgG showed major polypeptides of 66 kDa, 52.40 kDa and 20.72 kDa. The purified Immunoglobulin has been found to be immune-reactive by DID test and Western Blot analysis when treated against hyperimmune sera which was raised in rabbit.

Topik & Kata Kunci

Veterinary medicine

Penulis (4)

Manoj Kumar

Subhasis Batabyal

Shyamsundar Kesh

Saibal Chattopadhyay

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Kumar, M., Batabyal, S., Kesh, S., Chattopadhyay, S. (2013). PARTIAL PURIFICATION AND IMMUNE-BIOCHEMICAL CHARACTERIZATION OF DOG SERUM IMMUNOGLOBULIN G. http://www.animalmedicalresearch.org/Vol.3_Issue-1_June_2013/Manoj%20Kumar.pdf

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Tahun Terbit: 2013
Sumber Database: DOAJ
Akses: Open Access ✓