Isolation β-Stigmasterol from Teucrium hyrcanicum L., growing wild in Gilan Province

Background: The genus Teucrium (Lamiaceae) is comprised 340 species in widespread over the world. Remarkably, In the Flora Iranica this genus is represented by twelve species, three of which are endemic. The genus Teucrium are known for medicinal value, antibacterial, antioxidant, anti-inflamatory, antiseptic and antipyretic properties. Objective: Isolation of β-Stigmasterol from Teucrium hyrcanicum L. Methods: The aerial parts of Teucrium hyrcanicum were collected during the flowering stage from Rostamabad, Gilan Province, in July 2006 and then were extracted by chloroform and acetone (2:1) for 36 h. Crude extract was separated by adsorption chromatography over silica gel with elution by petroleum and ethylacetate. Final purification was performed by smaller chromatography. Results: A steroid named β - Stigmasterol were isolated and the structur of them were proved using spectral data consist of FTIR, UV, HRMS, also 1-DNMR (1HNMR, 13CNMR), 2-DNMR (HMQC, HMBC) and DEPT (90°,135°). Conclusion: β-Stigmasterol was characterized for the first time in Teucrium hyrcanicum.