DOAJ Open Access 2022

ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR

DAN ZGARDAN IRINA MITINA VALENTIN MITIN EMILIA BEHTA SILVIA RUBTOV +3 lainnya

Abstrak

Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit.

Topik & Kata Kunci

Penulis (8)

D

DAN ZGARDAN

I

IRINA MITINA

VALENTIN MITIN

E

EMILIA BEHTA

S

SILVIA RUBTOV

A

ALINA BOISTEAN

RODICA STURZA

M

MARIA MUNTEANU

Format Sitasi

ZGARDAN, D., MITINA, I., MITIN, V., BEHTA, E., RUBTOV, S., BOISTEAN, A. et al. (2022). ACETIC ACID BACTERIA DETECTION IN WINES BY REAL-TIME PCR. https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202202&vol=2&aid=5430

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Tahun Terbit
2022
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DOAJ
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Open Access ✓