DOAJ Open Access 2023

Enhancing DNA barcode reference libraries by harvesting terrestrial arthropods at the Smithsonian's National Museum of Natural History

Bernardo Santos Meredith Miller Margarita Miklasevskaja Jaclyn McKeown Niamh Redmond +22 lainnya

Abstrak

The use of DNA barcoding has revolutionised biodiversity science, but its application depends on the existence of comprehensive and reliable reference libraries. For many poorly known taxa, such reference sequences are missing even at higher-level taxonomic scales. We harvested the collections of the Smithsonian’s National Museum of Natural History (USNM) to generate DNA barcoding sequences for genera of terrestrial arthropods previously not recorded in one or more major public sequence databases. Our workflow used a mix of Sanger and Next-Generation Sequencing (NGS) approaches to maximise sequence recovery while ensuring affordable cost. In total, COI sequences were obtained for 5,686 specimens belonging to 3,737 determined species in 3,886 genera and 205 families distributed in 137 countries. Success rates varied widely according to collection data and focal taxon. NGS helped recover sequences of specimens that failed a previous run of Sanger sequencing. Success rates and the optimal balance between Sanger and NGS are the most important drivers to maximise output and minimise cost in future projects. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, the Global Genome Biodiversity Network Data Portal and the NMNH data portal.

Topik & Kata Kunci

Biology (General)

Penulis (27)

Bernardo Santos

Meredith Miller

Margarita Miklasevskaja

Jaclyn McKeown

Niamh Redmond

Jonathan Coddington

Jessica Bird

Scott Miller

Ashton Smith

Seán Brady

Matthew Buffington

M. Lourdes Chamorro

Torsten Dikow

Michael Gates

Paul Goldstein

Alexander Konstantinov

Robert Kula

Nicholas Silverson

M. Alma Solis

Stephanie deWaard

Suresh Naik

Nadya Nikolova

Mikko Pentinsaari

Sean Prosser

Jayme Sones

Evgeny Zakharov

Jeremy deWaard

Format Sitasi

APA MLA BibTeX

Santos, B., Miller, M., Miklasevskaja, M., McKeown, J., Redmond, N., Coddington, J. et al. (2023). Enhancing DNA barcode reference libraries by harvesting terrestrial arthropods at the Smithsonian's National Museum of Natural History. https://doi.org/10.3897/BDJ.11.e100904

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.3897/BDJ.11.e100904
Akses: Open Access ✓