DOAJ Open Access 2018

A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response

Yao Ma Yue-Ying Jiao Yun-Zhou Yu Nan Jiang Ying Hua +6 lainnya

Lihat Sumber DOI

Abstrak

Human respiratory syncytial virus (RSV) is the most significant cause of acute lower respiratory infection in children. However, there is no licensed vaccine available. Here, we investigated the effect of five or 20 copies of C-Class of CpG ODN (CpG-C) motif incorporated into a plasmid DNA vaccine encoding RSV fusion (F) glycoprotein on the vaccine-induced immune response. The addition of CpG-C motif enhanced serum binding and virus-neutralizing antibody responses in BALB/c mice immunized with the DNA vaccines. Moreover, mice vaccinated with CpG-modified vaccines, especially with the higher 20 copies, resulted in an enhanced shift toward a Th1-biased antibody and T-cell response, a decrease in pulmonary pathology and virus replication, and a decrease in weight loss after RSV challenge. This study suggests that CpG-C motif, cloned into the backbone of DNA vaccine encoding RSV F glycoprotein, functions as a built-in adjuvant capable of improving the efficacy of DNA vaccine against RSV infection.

Topik & Kata Kunci

Microbiology

Penulis (11)

Yao Ma

Yue-Ying Jiao

Yun-Zhou Yu

Nan Jiang

Ying Hua

Xiu-Juan Zhang

Yuan-Hui Fu

Xiang-Lei Peng

Yan-Peng Zheng

Larry J. Anderson

Jin-Sheng He

Format Sitasi

APA MLA BibTeX

Ma, Y., Jiao, Y., Yu, Y., Jiang, N., Hua, Y., Zhang, X. et al. (2018). A Built-In CpG Adjuvant in RSV F Protein DNA Vaccine Drives a Th1 Polarized and Enhanced Protective Immune Response. https://doi.org/10.3390/v10010038

Akses Cepat

Lihat di Sumber doi.org/10.3390/v10010038

Informasi Jurnal

Tahun Terbit: 2018
Sumber Database: DOAJ
DOI: 10.3390/v10010038
Akses: Open Access ✓