DOAJ Open Access 2024

Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay

Bastian Breiner Daniel M. Kainz Stefan Wagner Maxime Gavage Serhat Sahakalkan +3 lainnya

Abstrak

Food allergies are a severe burden for affected individuals and healthcare systems. To tackle the need for simple food allergen detection, we developed a system for the detection of the soy protein glycinin via a centrifugal microfluidics-assisted lateral flow immunoassay (LFIA). Glycinin is a complex allergen requiring extensive sample preparation. The presented workflow includes a manual denaturing extraction, followed by automated centrifugal microfluidic desalting, metering and detection via LFIA. The functionality of the microfluidic cassettes was tested on prototypes produced via microthermoforming before an injection molding tool was designed, which added a cylindrical lens to improve the readout. Overall, this system aims to aid in food allergen detection with high sensitivity and minimized manual steps.

Topik & Kata Kunci

General Works

Penulis (8)

Bastian Breiner

Daniel M. Kainz

Stefan Wagner

Maxime Gavage

Serhat Sahakalkan

Riccardo Marega

Felix von Stetten

Anna Klebes

Format Sitasi

APA MLA BibTeX

Breiner, B., Kainz, D.M., Wagner, S., Gavage, M., Sahakalkan, S., Marega, R. et al. (2024). Automated Allergen Sample Preparation and Detection via Centrifugal Microfluidic Lateral Flow Assay. https://doi.org/10.3390/proceedings2024097199

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Informasi Jurnal

Tahun Terbit: 2024
Sumber Database: DOAJ
DOI: 10.3390/proceedings2024097199
Akses: Open Access ✓