Imaging of Clear Cell Renal Carcinoma with Immune Checkpoint Targeting Aptamer-Based Probe
Abstrak
Immune checkpoint targeting immunotherapy has revolutionized the treatment of certain cancers in the recent years. Determination of the status of immune checkpoint expression in particular cancers may assist decision making. Here, we describe the development of a single-stranded aptamer-based molecular probe specifically recognizing human PD-L1. Target engaging aptamers are selected by iterative enrichment from a random ssDNA pool and the binding is characterized biochemically. Specificity and dose dependence is demonstrated in vitro in the cell culture using human kidney tumor cells (786-0), human melanoma cells (WM115 and WM266.4) and human glioblastoma LN18 cancer cells. The utility of the probe in vivo is demonstrated using two mouse tumor models, where we show that the probe exhibits excellent potential in imaging. We postulate that further development of the probe may allow universal imaging of different types of tumors depending on their PD-L1 status, which may find utility in cancer diagnosis.
Topik & Kata Kunci
Penulis (20)
Stanisław Malicki
Barbara Pucelik
Edyta Żyła
Małgorzata Benedyk-Machaczka
Wojciech Gałan
Anna Golda
Alicja Sochaj-Gregorczyk
Marta Kamińska
João Crispim Encarnação
Barbara Chruścicka
Hans-Peter Marti
Tony Jialiang Chen
Katarzyna Magiera-Mularz
Bartosz Zięba
Tad A. Holak
Janusz M. Dąbrowski
Anna Czarna
Joanna Kozieł
Piotr Mydel
Grzegorz Dubin
Akses Cepat
- Tahun Terbit
- 2022
- Sumber Database
- DOAJ
- DOI
- 10.3390/ph15060697
- Akses
- Open Access ✓