Construction of a High-Density Genetic Map for Pitaya Using the Whole Genome Resequencing Approach
Abstrak
Pitaya (<i>Hylocereus undatus</i>) is one of the most economic fleshy fruit tree crops. This study aimed at producing a high-density linkage genetic map of pitaya based on the whole genome resequencing (WGrS) approach. For this purpose, a bi-parental F1 population of 198 individuals was generated and genotyped by WGrS. High-quality polymorphic 6434 single polymorphism nucleotide (SNP) markers were extracted and used to construct a high-density linkage map. A total of 11 linkage groups were resolved as expected in accordance with the chromosome number. The map length was 14,128.7 cM with an average SNP interval of 2.2 cM. Homology with the sequenced reference genome was described, and the physical and genetic maps were compared with collinearity analysis. This linkage map in addition to the available genomic resources will help for quantitative trait mapping, evolutionary studies and marker-assisted selection in the important <i>Hylocereus</i> species.
Topik & Kata Kunci
Penulis (6)
Zhijiang Wu
Haiyan Deng
Guidong Liang
Xiaoying Ye
Yonghua Qin
Lifang Huang
Akses Cepat
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- 2021
- Sumber Database
- DOAJ
- DOI
- 10.3390/horticulturae7120534
- Akses
- Open Access ✓