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DOAJ Open Access 2025

Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase

Zheng Liu Ting Gao Shaoheng Bao Penggang Han Ge Yao +4 lainnya
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Abstrak

<i>Neo-allo</i>-ocimene is a monoterpene which could be applied in pesticides, fragrances, and sustainable polymers. In this study, we mined a terpene synthase, AgTPS40, from the transcriptome of celery leaf tissues. Through sequence and phylogenetic analysis, AgTPS40 was characterized as a monoterpene synthase. The <i>AgTPS40</i> gene was introduced into a heterologous mevalonate pathway hosted in <i>Escherichia coli</i> to enable terpene production. Gas chromatography–mass spectrometry analysis confirmed that AgTPS40 catalyzes the formation of <i>neo-allo</i>-ocimene, marking the first reported identification of a <i>neo-allo</i>-ocimene synthase. Subsequently, we optimized the fermentation conditions and achieved a yield of 933.35 mg/L in a 1 L shake flask, which represents the highest reported titer of <i>neo-allo</i>-ocimene to date. These results reveal the molecular basis of <i>neo-allo</i>-ocimene synthesis in celery and provide a sustainable way to obtain this compound.

Topik & Kata Kunci

Fermentation industries. Beverages. Alcohol

Penulis (9)

Z

Zheng Liu

T

Ting Gao

S

Shaoheng Bao

P

Penggang Han

G

Ge Yao

T

Tianyu Song

L

Longbao Zhu

C

Chang Chen

H

Hui Jiang

Format Sitasi

APA MLA BibTeX
Liu, Z., Gao, T., Bao, S., Han, P., Yao, G., Song, T. et al. (2025). Microbial Synthesis of <i>Neo-Allo</i>-Ocimene by Celery-Derived <i>Neo-Allo</i>-Ocimene Synthase. https://doi.org/10.3390/fermentation11030153

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Informasi Jurnal
Tahun Terbit
2025
Sumber Database
DOAJ
DOI
10.3390/fermentation11030153
Akses
Open Access ✓