In Vitro Schistosomicidal Activity and Molecular Modeling of Quercitrin and Afzelin Isolated from the Leaves of <i>Copaifera oblongifolia</i>

Neglected diseases significantly impact the world, and there is a lack of effective treatments, requiring therapeutic alternatives. Thus, the study of the phytochemical and schistosomicidal activity evaluation of <i>Copaifera oblongifolia</i> leaves’ crude extract was conducted. The quercitrin (<b>1</b>) and afzelin (<b>2</b>) were isolated from the crude extract. In the in vitro schistosomicidal activity test, the isolated compounds demonstrated promising results, with 75% mortality at a concentration of 12.5 µM after 72 h. Molecular docking calculations indicated that compounds <b>1</b> and <b>2</b> could potentially interact with the amino acids of the FAD binding site in the TGR enzyme, a crucial enzyme for the survival of <i>Schistosoma mansoni</i>. These interactions could have binding energies comparable to praziquantel, a preferred drug for treating schistosomiasis. Therefore, in silico and in vitro investigations are crucial for developing new studies that can reveal the antiparasitic potential of compounds of plant origin.