Muscle Enriched Lamin Interacting Protein (<i>Mlip</i>) Binds Chromatin and Is Required for Myoblast Differentiation
Abstrak
Muscle-enriched A-type lamin-interacting protein (<i>Mlip</i>) is a recently discovered Amniota gene that encodes proteins of unknown biological function. Here we report <i>Mlip</i>’s direct interaction with chromatin, and it may function as a transcriptional co-factor. Chromatin immunoprecipitations with microarray analysis demonstrated a propensity for <i>Mlip</i> to associate with genomic regions in close proximity to genes that control tissue-specific differentiation. Gel mobility shift assays confirmed that <i>Mlip</i> protein complexes with genomic DNA. Blocking <i>Mlip</i> expression in C2C12 myoblasts down-regulates myogenic regulatory factors (MyoD and MyoG) and subsequently significantly inhibits myogenic differentiation and the formation of myotubes. Collectively our data demonstrate that <i>Mlip</i> is required for C2C12 myoblast differentiation into myotubes. <i>Mlip</i> may exert this role as a transcriptional regulator of a myogenic program that is unique to amniotes.
Topik & Kata Kunci
Penulis (3)
Elmira Ahmady
Alexandre Blais
Patrick G. Burgon
Akses Cepat
- Tahun Terbit
- 2021
- Sumber Database
- DOAJ
- DOI
- 10.3390/cells10030615
- Akses
- Open Access ✓