DOAJ Open Access 2023

In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips

Mariola Dreger Aleksandra Deja Milena Szalata Ryszard Słomski

Abstrak

Synthetic seed technology and cold storage methods provide genetic uniformity, paving the way for pest- and disease-free plants that are easy to handle. The aim of this study was to develop protocols for the cold storage of nonencapsulated and alginate-capsulated explants of <i>Cannabis sativa</i> L. Axillary shoot tips derived from in vitro-grown plants that were used as explants and stored for up to 9 months at 4 °C in the dark. Somatic seeds were produced in 3% sodium alginate and Murashige and Skoog (MS) medium salt and stored for up to 3 months. After 6 months of cold storage, the highest regrowth of 45% was recorded for the nonencapsulated explants. The recovery of somatic seeds was 90% under the same storage condition after 3 months. Well-developed, regenerated plants from encapsulated explants successfully acclimatized.

Topik & Kata Kunci

Plant ecology Animal biochemistry Biology (General)

Penulis (4)

Mariola Dreger

Aleksandra Deja

Milena Szalata

Ryszard Słomski

Format Sitasi

APA MLA BibTeX

Dreger, M., Deja, A., Szalata, M., Słomski, R. (2023). In Vitro Preservation of Somatic Seeds and Nonencapsulated Hemp Shoot Tips. https://doi.org/10.3390/IECAG2023-15146

Akses Cepat

PDF tidak tersedia langsung

Cek di sumber asli →

Lihat di Sumber doi.org/10.3390/IECAG2023-15146

Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.3390/IECAG2023-15146
Akses: Open Access ✓