Ultrasensitive Raman Spectroscopy-Based Virus Detection Using Glycan-Coated Plasmonic Substrates
Abstrak
Hepatitis viral infections are the most common cause of hepatitis liver disease, which eventually leads to cancer and fibrosis if not detected early. Therefore, early detection would allow for preventive and therapeutic actions. Here, a surface-enhanced Raman spectroscopy (SERS)-based biosensor was developed using plasmonic molybdenum trioxide quantum dots (MoO<sub>3</sub>-QDs) as the SERS substrates. The nanostructured substrate of MoO<sub>3</sub>-QDs was functionalized with a proteoglycan (syndecan-1) as a novel bioreceptor for the target hepatitis E virus (HEV). The innovative biodetection system achieved a detection limit of 1.05 fg/mL for the tested HEV target (ORF2), indicating superb clinically relevant sensitivity and performance. The designed biosensing system incorporating a glycan motif as a bioreceptor instead of the conventional antibodies or aptamers presents new insights for the ultrasensitive detection of HEV and other infectious viruses.
Topik & Kata Kunci
Penulis (2)
Ojodomo J. Achadu
Enoch Y. Park
Akses Cepat
- Tahun Terbit
- 2023
- Sumber Database
- DOAJ
- DOI
- 10.3390/CSAC2023-14922
- Akses
- Open Access ✓