A comparative assessment of GPX-1 expression and histomorphometry evaluation in IBD vaccinated and supplemented broiler

This article was designed to investigate the bursa of Fabricius (BF) morphometric and effects of glutathione peroxidase enzyme (GPX-1) in infectious bursal disease vaccinated (IBDV) broiler supplemented with selenium-nanomaterials (Se-NPs). Ninety-six one-day-old of the Ross 308 broilers were assigned into E1, E2, E3, and E4 of 24 chicks each; those of E1 served as control, E2 vaccinated with an intermediate strain of IBD at 12 and 20 days old through the eye drop, E3 received a mix of Se-NPs and the vaccine, and E4 was supplemented with Se-NPs (0.3mg/kg). At 19, 26, and 42 days of age, the diameters of the bursa of Fabricius were measured, and liver tissue was sampled to determine the reverse transcription polymerase chain reaction (RT-PCR) of the Glutathione peroxidase enzyme. Results indicated that group E4 (selenium-Nanoparticles group) significantly increased the bursal morphometric at 26 days old, and E3 increased the morphometric of the bursa Fabricius at 42 days old, as well as supplementation of Se-NPs significantly up-regulated RT-PCR of GPX-1 at 26 and 42 days old. The highest gene expression was in the selenium-nanoparticle group at 26 and 42 days old, in contrast to the other groups. Based on this finding, it can be concluded that nanomaterials improved the morphology of immune organs and enzyme peroxidase activity in vaccinated broilers with the Gumboro vaccine.