DOAJ Open Access 2023

In vivo loss of tumorigenicity in a patient-derived orthotopic xenograft mouse model of ependymoma

Jacqueline P. Whitehouse Jacqueline P. Whitehouse Hilary Hii Chelsea Mayoh Chelsea Mayoh +40 lainnya

Abstrak

IntroductionEpendymomas (EPN) are the third most common malignant brain cancer in children. Treatment strategies for pediatric EPN have remained unchanged over recent decades, with 10-year survival rates stagnating at just 67% for children aged 0-14 years. Moreover, a proportion of patients who survive treatment often suffer long-term neurological side effects as a result of therapy. It is evident that there is a need for safer, more effective treatments for pediatric EPN patients. There are ten distinct subgroups of EPN, each with their own molecular and prognostic features. To identify and facilitate the testing of new treatments for EPN, in vivo laboratory models representative of the diverse molecular subtypes are required. Here, we describe the establishment of a patient-derived orthotopic xenograft (PDOX) model of posterior fossa A (PFA) EPN, derived from a metastatic cranial lesion.MethodsPatient and PDOX tumors were analyzed using immunohistochemistry, DNA methylation profiling, whole genome sequencing (WGS) and RNA sequencing.ResultsBoth patient and PDOX tumors classified as PFA EPN by methylation profiling, and shared similar histological features consistent with this molecular subgroup. RNA sequencing revealed that gene expression patterns were maintained across the primary and metastatic tumors, as well as the PDOX. Copy number profiling revealed gains of chromosomes 7, 8 and 19, and loss of chromosomes 2q and 6q in the PDOX and matched patient tumor. No clinically significant single nucleotide variants were identified, consistent with the low mutation rates observed in PFA EPN. Overexpression of EZHIP RNA and protein, a common feature of PFA EPN, was also observed. Despite the aggressive nature of the tumor in the patient, this PDOX was unable to be maintained past two passages in vivo.DiscussionOthers who have successfully developed PDOX models report some of the lowest success rates for EPN compared to other pediatric brain cancer types attempted, with loss of tumorigenicity not uncommon, highlighting the challenges of propagating these tumors in the laboratory. Here, we discuss our collective experiences with PFA EPN PDOX model generation and propose potential approaches to improve future success in establishing preclinical EPN models.

Topik & Kata Kunci

Neoplasms. Tumors. Oncology. Including cancer and carcinogens

Penulis (45)

Jacqueline P. Whitehouse

Hilary Hii

Chelsea Mayoh

Marie Wong

Pamela Ajuyah

Paulette Barahona

Louise Cui

Hetal Dholaria

Christine L. White

Molly K. Buntine

Jacob Byrne

Keteryne Rodrigues da Silva

Meegan Howlett

Emily J. Girard

Maria Tsoli

David S. Ziegler

Jason M. Dyke

Sharon Lee

Paul G. Ekert

Mark J. Cowley

Nicholas G. Gottardo

Raelene Endersby

Format Sitasi

APA MLA BibTeX

Whitehouse, J.P., Whitehouse, J.P., Hii, H., Mayoh, C., Mayoh, C., Wong, M. et al. (2023). In vivo loss of tumorigenicity in a patient-derived orthotopic xenograft mouse model of ependymoma. https://doi.org/10.3389/fonc.2023.1123492

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.3389/fonc.2023.1123492
Akses: Open Access ✓