DOAJ Open Access 2022

Growth Hormone Receptor Controls Adipogenic Differentiation of Chicken Bone Marrow Mesenchymal Stem Cells by Affecting Mitochondrial Biogenesis and Mitochondrial Function

Changbin Zhao Changbin Zhao Changbin Zhao Changbin Zhao Bowen Hu +43 lainnya

Abstrak

Growth hormone receptor (GHR) can activate several signaling pathways after binding to growth hormone (GH) to regulate cell growth and development. Sex-linked dwarf (SLD) chickens, normal protein functions are prevented because of exon mutations in the GHR gene, have more severe fat deposition. However, the specific molecular mechanisms responsible for this phenotype remains unclear. We therefore investigated the effect of the GHR gene on adipogenic differentiation of chicken bone marrow mesenchymal stem cells (BMSCs). We found that bone marrow fat deposition was more severe in SLD chickens compared to normal chickens, and the expression of genes related to adipogenic differentiation was enhanced in SLD chicken BMSCs. We also detected enhanced mitochondrial function of BMSCs in SLD chickens. In vitro, overexpression of GHR in chicken BMSCs increased mitochondrial membrane potential but decreased reactive oxygen and ATP contents, oxidative phosphorylation complex enzyme activity, and mitochondrial number. Expression of genes associated with mitochondrial biogenesis and function was repressed during adipogenic differentiation in chicken BMSCs, the adipogenic differentiation capacity of chicken BMSCs was also repressed. With knockdown of GHR, opposite results were observed. We concluded that GHR inhibited adipogenic differentiation of chicken BMSCs by suppressing mitochondrial biogenesis and mitochondrial function.

Topik & Kata Kunci

Biology (General)

Penulis (48)

Changbin Zhao

Bowen Hu

Zhiying Liao

Haohui Wei

Yongxia Zhao

Jinping Liang

Wen Luo

Qinghua Nie

Qingbin Luo

Dexiang Zhang

Xiquan Zhang

Hongmei Li

Format Sitasi

APA MLA BibTeX

Zhao, C., Zhao, C., Zhao, C., Zhao, C., Hu, B., Hu, B. et al. (2022). Growth Hormone Receptor Controls Adipogenic Differentiation of Chicken Bone Marrow Mesenchymal Stem Cells by Affecting Mitochondrial Biogenesis and Mitochondrial Function. https://doi.org/10.3389/fcell.2022.827623

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Informasi Jurnal

Tahun Terbit: 2022
Sumber Database: DOAJ
DOI: 10.3389/fcell.2022.827623
Akses: Open Access ✓