Bookmark Saya

DOAJ Open Access 2016

Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties

G. I. Alatortseva A. V. Sidorov L. N. Nesterenko L. N. Luhverchik I. I. Amiantova +7 lainnya
Lihat Sumber DOI

Abstrak

We made recombinant antigen GE containing fragment of VZV glycoprotein E (Gly48 - Glu135) fused to E. coli beta-galactosidase and confirmed its antigen specificity by Western blotting and competitive-inhibition enzyme immunoassay (EIA) in comparison with commercial analogues and natural viral antigens. We showed interaction of recombinant GE protein with IgG antibodies from rabbits immunized by vaccine viral strain. GE protein also specifically reacted in ELISA with 66% of sera from zoster patients and 35% of sera from control groups including sera containing antibodies to other herpes viruses, sera from healthy donors, and sera from patients with different forms of intestinal disorders. Consequently, we demonstrated possibility of application of our recombinant GE VZV as antigen for diagnostics and research use.

Topik & Kata Kunci

Epistemology. Theory of knowledge

Penulis (12)

G

G. I. Alatortseva

A

A. V. Sidorov

L

L. N. Nesterenko

L

L. N. Luhverchik

I

I. I. Amiantova

V

V. V. Docenko

P

Phong Pham Huy

A

A. V. Milovanova

O

O. B. Vylivannaya

A

A. C. Kazanova

V

V. V. Lavrov

V

V. V. Zverev

Format Sitasi

APA MLA BibTeX
Alatortseva, G.I., Sidorov, A.V., Nesterenko, L.N., Luhverchik, L.N., Amiantova, I.I., Docenko, V.V. et al. (2016). Recombinant Analogue of Varicella Zoster Virus Glycoprotein E: Cloning, Expression and Studying of AntigEn Properties. https://doi.org/10.31631/2073-3046-2016-15-1-77-85

Akses Cepat

PDF tidak tersedia langsung

Cek di sumber asli →
Lihat di Sumber doi.org/10.31631/2073-3046-2016-15-1-77-85
Informasi Jurnal
Tahun Terbit
2016
Sumber Database
DOAJ
DOI
10.31631/2073-3046-2016-15-1-77-85
Akses
Open Access ✓