Detection of inhibitor residues in milk

The article explores methods for detecting residues of inhibitory substances (ISRs) in raw cow's milk, with a particular focus on antibiotics that may enter milk as a result of treating animals. The presence of ISRs in milk has a significant impact on both consumer health and the efficiency of the dairy industry, as antibiotics can interfere with milk processing technologies. The study utilized various techniques to analyze milk samples collected from dairy farms in the Kursk region, including selective rapid tests such as Twinsensor BT and SNAP b-lactam, broad-spectrum tests like Delvotest T and Eclipse, as well as enzyme-linked immunosorbent assay (ELISA). The results showed that Twinsensor BT demonstrated high accuracy in detecting beta-lactam and tetracycline antibiotic residues, identifying inhibitory substances in 16.7% of milk samples. The SNAP b-lactam test confirmed these findings, detecting 13.3% of positive samples. Broad-spectrum tests like Delvotest T and Eclipse exhibited even greater sensitivity, with positive results obtained in 20% and 16.7% of samples, respectively. The ELISA confirmed the presence of antibiotics such as streptomycin and penicillin, detecting residues in 13.3% of samples. Overall, approximately 20% of the tested milk samples contained ISR residues, highlighting the need for stricter control over the use of antibiotics in veterinary practice and regular monitoring of dairy products at all stages of production. The importance of using multiple detection methods to obtain the most accurate data on milk contamination with inhibitory substances is also a key conclusion of the study. The results indicate a high prevalence of antibiotic residues in milk, which may adversely affect its processing and safety. The Delvotest T and Eclipse tests were found to be the most effective in detecting a wide range of inhibitory substances, confirming their importance for monitoring. The data emphasize the need for stricter measures to control the use of veterinary drugs in dairy farming.