DOAJ Open Access 2017

A SENSITIVE ANALYTICAL (RP-HPLC-PDA, UV/VIS) METHOD FOR THE DETERMINATION OF NEWLY SYNTHESIZED N-ISONICOTINOYL-N´-(3-FLUOROBENZAL)HYDRAZONE (SH2) IN AQUEOUS PHASE

Zvezdelina YANEVA Nedyalka GEORGIEVA

Abstrak

The purpose of the present study was to develop a simple, rapid and reproducible analytical method for the determination of N-isonicotynoyl-N´-(3-fluorobenzal)hydrazone (SH2) - a synthesized by us halogenated isoniazid derivative with high tuberculostatic activity, in aqueous phase, on the basis of RP-HPLC-PDA and UV/VIS spectrophotometric investigations. Despite of the high linearity (R2 0.9984) of the UV/VIS spectrophotometric method applied, the significantly higher LOQ and LOD values indicated its unsuitability for detection and quantification of low N-isonicotynoyl-N´-(3-fluorobenzal)hydrazone concentrations (< 10 µg/mL). The proposed RP-HPLC-PDA method with mobile phase ACN/phosphate buffer (60:40, v/v) offered short retention time (3.1 min), high precision (RSD 3.50 %) and linearity (R2 0.9898). It characterized with satisfactory LOD (0.346 µg/mL) and LOQ (1.05 µg/mL) values and allowed the qualitative detection of SH2 E/Z-isomer.

Topik & Kata Kunci

Chemistry

Penulis (2)

Zvezdelina YANEVA

Nedyalka GEORGIEVA

Format Sitasi

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YANEVA, Z., GEORGIEVA, N. (2017). A SENSITIVE ANALYTICAL (RP-HPLC-PDA, UV/VIS) METHOD FOR THE DETERMINATION OF NEWLY SYNTHESIZED N-ISONICOTINOYL-N´-(3-FLUOROBENZAL)HYDRAZONE (SH2) IN AQUEOUS PHASE. https://doi.org/10.24193/subbchem.2017.2.15

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Informasi Jurnal

Tahun Terbit: 2017
Sumber Database: DOAJ
DOI: 10.24193/subbchem.2017.2.15
Akses: Open Access ✓