DOAJ Open Access 2015

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

Nathan Wales Christian Carøe Marcela Sandoval-Velasco Cristina Gamba Ross Barnett +4 lainnya

Abstrak

An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples. To investigate the behavior of this method, we generated ssDNA and conventional double-stranded DNA (dsDNA) libraries from 23 ancient and historic plant and animal specimens. We found ssDNA library preparation substantially increased endogenous content when dsDNA libraries contained <3% endogenous DNA, but this enrichment is less pronounced when dsDNA preparations successfully recover short endogenous DNA fragments (mean size < 70 bp). Our findings can help researchers determine when to utilize the time- and resource-intensive ssDNA library preparation method.

Topik & Kata Kunci

Biology (General)

Penulis (9)

Nathan Wales

Christian Carøe

Marcela Sandoval-Velasco

Cristina Gamba

Ross Barnett

José Alfredo Samaniego

Jazmín Ramos Madrigal

Ludovic Orlando

M. Thomas P. Gilbert

Format Sitasi

APA MLA BibTeX

Wales, N., Carøe, C., Sandoval-Velasco, M., Gamba, C., Barnett, R., Samaniego, J.A. et al. (2015). New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA. https://doi.org/10.2144/000114364

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Informasi Jurnal

Tahun Terbit: 2015
Sumber Database: DOAJ
DOI: 10.2144/000114364
Akses: Open Access ✓