Electrochemical detection of Gonadotropin-releasing hormone agonists for uterine fibroids based on Poly(L-Serine)/Ag NPs/GO nanocomposite modified glassy carbon electrode

In this work, an electrochemical sensor of Gonadotropin-releasing hormone (GnRH) agonists was proposed by deposition of the conductive nanocomposite of Ag and graphene oxide on glassy carbon electrode (Ag-GO/GCE) and electropolymerization of poly(L-Serine) on nanocomposite (p-L-serine/Ag-GO/GCE). Because of excellent conductivity between Ag nanoparticles and graphene oxide nansheets, and synergic catalytic effect of Ag-GO nanocomposite and the conducting polymer, the developed GnRH sensor provides a highly porous surface and a larger effective surface area, which facilitates electron transfer and promotes the electro-catalytic performance. p-L-serine/Ag-GO/GCE exhibited good electro-catalytic and selectivity with a sensitivity of 0.74036 μA/μgml-1, a linear range of 1 to 15×107 μg/ml, and a low detection limit of 20 pg/ml (S/N = 3). Moreover, the p-L-serine/Ag-GO/GCE was successfully applied for the determination of GnRH in real blood serum of patients aged 40-50 years old who administrated GnRH for treatment of uterine fibroids and the obtained RSD≤4.20% was demonstrated to good accuracy of p-L-serine/Ag-GO/GCE for the determination of GnRH in patient blood serum and in clinical applications.