DOAJ Open Access 2013

Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells

Yuki Ota Takuya Kawanai Ryo Watanabe Akira Nishimura Yukio Ago +2 lainnya

Abstrak

Abstract.: Nitric oxide (NO) induces cytotoxicity in neuronal and glial cells via activation of the Na+/Ca2+ exchanger (NCX). This study examined the role of the predominant brain-specific NCX splice variant NCX1.5 in NO-induced cytotoxicity in the HEK293 cell expression system. Cells were transfected with the plasmid construct pcDNA3.1/V5-His containing full-length rat NCX1.5 cDNA. There was no difference in the cytotoxic effects of the NO donors sodium nitroprusside and S-nitroso-N-acetylpenicillamine between control and transfected cells. These results suggest that NO cytotoxicity is not dependent on NCX1.5. Keywords:: Na+/Ca2+ exchanger, nitric oxide, cytotoxicity

Topik & Kata Kunci

Therapeutics. Pharmacology

Penulis (7)

Yuki Ota

Takuya Kawanai

Ryo Watanabe

Akira Nishimura

Yukio Ago

Kazuhiro Takuma

Toshio Matsuda

Format Sitasi

APA MLA BibTeX

Ota, Y., Kawanai, T., Watanabe, R., Nishimura, A., Ago, Y., Takuma, K. et al. (2013). Effect of Overexpression of the Brain-Specific Na+/Ca2+ Exchanger Splice Variant NCX1.5 on NO Cytotoxicity in HEK293 Cells. https://doi.org/10.1254/jphs.13012SC

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Informasi Jurnal

Tahun Terbit: 2013
Sumber Database: DOAJ
DOI: 10.1254/jphs.13012SC
Akses: Open Access ✓