Quality Detection method on cleaning quality of silicone tube in phacoemulsification

ObjectiveThe present study intends to observe the cleaning effect of different detection methods in cleaning silicone tubes used in phacoemulsification.MethodsA total of 100 silicone tubes were selected randomly after surgery. The silicone tubes were retained for ≤2 hours after surgery, and then washed with a high-pressure water gun at a flow rate of 12~14 ml/s. Adenosine Triphosphate (ATP) detection and quantitation of residual protein were performed on the samples before cleaning and after washing for 30 s, 40 s, and 50 s, respectively, including the sample surface and the water after cleaning.ResultsAccording to the results before and after cleaning the silicone tube, there are significant differences in three methods of quantitation of residual protein, ATP detection in water sample, and ATP detection in sample surface (c2=8.6, P<0.05), while having no difference between the three methods after washing for 30 s, 40 s and 50 s, respectively (c2=4.918 and 5.571, P>0.05). A comparison of the means of ATP detection in water samples showed significant differences between rinses 30 s/40 s and 30 s/50 s. (Z=-7.45 and -0.08, P<0.05); pairwise contrast of ATP detection in sample surface for rinsing 30 s/40 s, 40 s/50 s, and 30 s/50 s showed significant differences (Z=3.64, 14.92, and 25.86, P<0.05). The quantitation of residual protein in silicone tubes showed pass rates of 84%, 100%, and 100% for 30 s, 40 s, and 50 s, respectively.ConclusionQuantitation of residual protein, ATP detection in water sample, and ATP detection in sample surface are available for monitoring the cleaning quality of silicone tube. The tube should be cleaned at a 12~14 ml/s flow rate and a washing time of ≥50 s.