DOAJ Open Access 2025

HELLS is required for maintaining proper DNA modification at human satellite repeats

Philine Guckelberger Leah Haut Rosaria Tornisiello Helene Kretzmer Alexander Meissner

Abstrak

Abstract DNA methylation regulation involves multi-layered chromatin interactions that require remodeling proteins like the helicase, lymphoid-specific (HELLS). Here, we generate HELLS and DNA methyltransferase 3A and B (DNMT3A/B) knockout human pluripotent stem cells and report telomere-to-telomere maps of whole genome bisulfite sequencing data combined with ATAC-sequencing. Disrupting HELLS induces a global loss of DNA methylation that is distinct from the DNMTs, in particular over peri/centromeric satellite repeats as defined in the telomere-to-telomere genome assembly. However, HELLS appears dispensable for local enhancer remodeling and the potential to differentiate into the three embryonic germ layers. Taken together, our results further clarify the genomic targets and role of HELLS in human cells.

Topik & Kata Kunci

Biology (General) Genetics

Penulis (5)

Philine Guckelberger

Leah Haut

Rosaria Tornisiello

Helene Kretzmer

Alexander Meissner

Format Sitasi

APA MLA BibTeX

Guckelberger, P., Haut, L., Tornisiello, R., Kretzmer, H., Meissner, A. (2025). HELLS is required for maintaining proper DNA modification at human satellite repeats. https://doi.org/10.1186/s13059-025-03681-9

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Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1186/s13059-025-03681-9
Akses: Open Access ✓