DOAJ Open Access 2025

Meta-surface plasmon resonance microscopy for sensitive, high-throughput, and long-term label-free analysis of cell adhesion dynamics

Mingqian Chen Wen Li Taoyu Hu Jiaxiang Chang Youqian Chen +3 lainnya

Abstrak

Abstract Quantitative, label-free monitoring of dynamic cell adhesion remains challenging. Meta-Surface Plasmon Resonance Microscopy (Meta-SPRM) is a novel platform integrating bright-field microscopy with engineered Meta-SPR nanocup arrays. This system simultaneously acquires bright-field images and Meta-SPRM signals, enabling their computational separation and co-analysis to provide multifaceted insights into cell-substrate interactions. Meta-SPRM offers sensitive, high-throughput, and long-term label-free quantification of cell adhesion strength and distribution. It captures dynamic processes like cell spreading and migration at micrometer lateral resolution. Notably, Meta-SPRM signals spatially correlate with key focal adhesion proteins (Integrin-β1, Vinculin), and an intrinsic intracellular signal polarity correlates with cell migration direction. Meta-SPRM provides a powerful, label-free tool for dynamic cell adhesion studies, overcoming limitations of traditional methods. Graphical abstract

Topik & Kata Kunci

Biotechnology Medical technology

Penulis (8)

Mingqian Chen

Wen Li

Taoyu Hu

Jiaxiang Chang

Youqian Chen

Yihui Yang

Gang Logan Liu

Wenjun Hu

Format Sitasi

APA MLA BibTeX

Chen, M., Li, W., Hu, T., Chang, J., Chen, Y., Yang, Y. et al. (2025). Meta-surface plasmon resonance microscopy for sensitive, high-throughput, and long-term label-free analysis of cell adhesion dynamics. https://doi.org/10.1186/s12951-025-03828-7

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Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1186/s12951-025-03828-7
Akses: Open Access ✓