DOAJ Open Access 2016

Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis

Ana C Santos Leite da Silva Nadia Woitovich Valetti María E Brassesco José A Teixeira Guillermo Picó

Abstrak

This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gum–alginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.

Topik & Kata Kunci

Physical and theoretical chemistry

Penulis (5)

Ana C Santos Leite da Silva

Nadia Woitovich Valetti

María E Brassesco

José A Teixeira

Guillermo Picó

Format Sitasi

APA MLA BibTeX

Silva, A.C.S.L.d., Valetti, N.W., Brassesco, M.E., Teixeira, J.A., Picó, G. (2016). Adsorption of peroxidase from L onto alginate–guar gum matrix: Kinetic, equilibrium and thermodynamic analysis. https://doi.org/10.1177/0263617416659287

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Informasi Jurnal

Tahun Terbit: 2016
Sumber Database: DOAJ
DOI: 10.1177/0263617416659287
Akses: Open Access ✓