A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography
Abstrak
Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine `non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.
Topik & Kata Kunci
Penulis (17)
Raphaël de Wijn
Oliver Hennig
Jennifer Roche
Sylvain Engilberge
Kevin Rollet
Pablo Fernandez-Millan
Karl Brillet
Heike Betat
Mario Mörl
Alain Roussel
Eric Girard
Christoph Mueller-Dieckmann
Gavin C. Fox
Vincent Olieric
José A. Gavira
Bernard Lorber
Claude Sauter
Format Sitasi
Akses Cepat
- Tahun Terbit
- 2019
- Sumber Database
- DOAJ
- DOI
- 10.1107/S2052252519003622
- Akses
- Open Access ✓