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DOAJ Open Access 2023

Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells

Danang Dwi Cahyadi Tomoko Warita Nanami Irie Kana Mizoguchi Jiro Tashiro +2 lainnya
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Abstrak

ABSTRACTNormalization is a crucial step in gene expression analysis to avoid misinterpretation. Reverse transcription-quantitative polymerase chain reaction was used to measure the expression of 10 candidate housekeeping genes in non-differentiated (ND) and differentiated (DI) 3T3-L1 cells on days 5 and 10. We used geNorm, NormFinder, BestKeeper, RefFinder, and the ∆Ct method to evaluate expression stability. The findings revealed that (1) the expression levels of the reference genes changed over time, even in non-differentiating cells, and (2) peptidylprolyl isomerase A (Ppia) and TATA box-binding protein (Tbp) were stable reference genes for 10 days in both undifferentiated and differentiated 3T3-L1 cells. Notably, the expression of known reference genes in non-differentiating cells was altered throughout the experiment.

Topik & Kata Kunci

Diseases of the endocrine glands. Clinical endocrinology Cytology Physiology

Penulis (7)

D

Danang Dwi Cahyadi

T

Tomoko Warita

N

Nanami Irie

K

Kana Mizoguchi

J

Jiro Tashiro

Y

Yoshinao Z. Hosaka

K

Katsuhiko Warita

Format Sitasi

APA MLA BibTeX
Cahyadi, D.D., Warita, T., Irie, N., Mizoguchi, K., Tashiro, J., Hosaka, Y.Z. et al. (2023). Housekeeping gene expression variability in differentiating and non-differentiating 3T3-L1 cells. https://doi.org/10.1080/21623945.2023.2235081

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Informasi Jurnal
Tahun Terbit
2023
Sumber Database
DOAJ
DOI
10.1080/21623945.2023.2235081
Akses
Open Access ✓