DOAJ Open Access 2021

Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis

Steffi Bredemeyer Lutz Roewer Sascha Willuweit

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Abstrak

To evaluate the promising advantages of massively parallel sequencing (MPS) in our casework, we analysed a total of 33 Y-chromosomal short tandem repeats (Y-STRs) with traditional capillary electrophoresis (CE) and 25 Y-STRs using the newer MPS technology. We studied the outcome of both technologies in 64 father-son pairs using stock and custom-designed kits. Current MPS technology confirmed the 13 mutational events observed with CE and improved our understanding of the complex nature of STR mutations. By detecting isometric sequence variants between unrelated males, we show that sequencing Y-STRs using MPS can boost discrimination power.

Topik & Kata Kunci

Criminal law and procedure Public aspects of medicine

Penulis (3)

Steffi Bredemeyer

Lutz Roewer

Sascha Willuweit

Format Sitasi

APA MLA BibTeX

Bredemeyer, S., Roewer, L., Willuweit, S. (2021). Next generation sequencing of Y-STRs in father-son pairs and comparison with traditional capillary electrophoresis. https://doi.org/10.1080/20961790.2021.1898078

Akses Cepat

Lihat di Sumber doi.org/10.1080/20961790.2021.1898078

Informasi Jurnal

Tahun Terbit: 2021
Sumber Database: DOAJ
DOI: 10.1080/20961790.2021.1898078
Akses: Open Access ✓