Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene
Abstrak
Abstract Stimulated emission depletion (STED) microscopy, a key optical super-resolution imaging method, has extended our ability to view details to resolution levels of tens of nanometers. Its resolution depends on fluorophore de-excitation efficiency, and increases with depletion laser power. However, high-power irradiation permanently turns off the fluorescence due to photo-bleaching of the fluorophores. As a result, there is a trade-off between spatial resolution and imaging time. Here, we overcome this limitation by introducing reactivatable STED (ReSTED) based on the photophysical properties of the nanographene dibenzo[hi,st]ovalene (DBOV). In contrast to the photo-induced decomposition of other fluorophores, the fluorescence of DBOV is only temporarily deactivated and can be reactivated by near-infrared light (including the 775 nm depletion beam). As a result, this fluorophore allows for hours-long, high-resolution 3D STED imaging, greatly expanding the applications of STED microscopy.
Topik & Kata Kunci
Penulis (20)
Qiqi Yang
Antonio Virgilio Failla
Petri Turunen
Ana Mateos-Maroto
Meiyu Gai
Werner Zuschratter
Sophia Westendorf
Márton Gelléri
Qiang Chen
Goudappagouda
Hao Zhao
Xingfu Zhu
Svenja Morsbach
Marcus Scheele
Wei Yan
Katharina Landfester
Ryota Kabe
Mischa Bonn
Akimitsu Narita
Xiaomin Liu
Akses Cepat
- Tahun Terbit
- 2025
- Sumber Database
- DOAJ
- DOI
- 10.1038/s41467-025-56401-z
- Akses
- Open Access ✓