The role of the essential GTPase ObgE in regulating lipopolysaccharide synthesis in Escherichia coli
Abstrak
Abstract During growth, cells need to synthesize and expand their envelope, a process that requires careful regulation. Here, we show that the GTPase ObgE of E. coli contributes to the regulation of lipopolysaccharide (LPS) synthesis, an essential component of the Gram-negative outer membrane. Using a dominant-negative mutant (named ‘ObgE*’), we show a direct interaction between ObgE and LpxA, which catalyzes the first step in LPS synthesis. This interaction is enhanced by the mutation in ObgE* which, when bound to GTP, leads to inhibition of LpxA, decreased LPS synthesis, and cell death. Although wild-type ObgE does not exert the same strong effects as ObgE* on LpxA or LPS synthesis, our data indicate that ObgE participates in the regulation of cell envelope synthesis in E. coli. Because ObgE also influences other cellular functions (i.e., ribosome assembly, DNA replication, etc.), it seems increasingly plausible that this GTPase coordinates several processes to finetune cell growth.
Topik & Kata Kunci
Penulis (12)
Liselot Dewachter
Babette Deckers
Israel Mares-Mejía
Elen Louwagie
Silke Vercauteren
Paul Matthay
Simon Brückner
Anna-Maria Möller
Franz Narberhaus
Sibylle C. Vonesch
Wim Versées
Jan Michiels
Akses Cepat
- Tahun Terbit
- 2024
- Sumber Database
- DOAJ
- DOI
- 10.1038/s41467-024-53980-1
- Akses
- Open Access ✓