Precise immunofluorescence canceling for highly multiplexed imaging to capture specific cell states
Abstrak
Abstract Cell states are regulated by the response of signaling pathways to receptor ligand-binding and intercellular interactions. High-resolution imaging has been attempted to explore the dynamics of these processes and, recently, multiplexed imaging has profiled cell states by achieving a comprehensive acquisition of spatial protein information from cells. However, the specificity of antibodies is still compromised when visualizing activated signals. Here, we develop Precise Emission Canceling Antibodies (PECAbs) that have cleavable fluorescent labeling. PECAbs enable high-specificity sequential imaging using hundreds of antibodies, allowing for reconstruction of the spatiotemporal dynamics of signaling pathways. Additionally, combining this approach with seq-smFISH can effectively classify cells and identify their signal activation states in human tissue. Overall, the PECAb system can serve as a comprehensive platform for analyzing complex cell processes.
Topik & Kata Kunci
Penulis (19)
Kosuke Tomimatsu
Takeru Fujii
Ryoma Bise
Kazufumi Hosoda
Yosuke Taniguchi
Hiroshi Ochiai
Hiroaki Ohishi
Kanta Ando
Ryoma Minami
Kaori Tanaka
Taro Tachibana
Seiichi Mori
Akihito Harada
Kazumitsu Maehara
Masao Nagasaki
Seiichi Uchida
Hiroshi Kimura
Masashi Narita
Yasuyuki Ohkawa
Akses Cepat
- Tahun Terbit
- 2024
- Sumber Database
- DOAJ
- DOI
- 10.1038/s41467-024-47989-9
- Akses
- Open Access ✓