DOAJ Open Access 2025

Protocol for culturing olfactory epithelium organoids supporting neuronal differentiation

Juliana Gutschow Gameiro Jussi Virtanen Liam Lee James E. Schwob Marco Aurélio Fornazieri +1 lainnya

Abstrak

Summary: The olfactory epithelium (OE) contains stem cells capable of generating olfactory sensory neurons throughout life, making it a valuable model for studying epithelial neurogenesis. We present a protocol to develop a highly robust 3D mouse organoid model from OE cells. We describe a workflow for dissecting murine OE and subsequent organoid culturing. We also provide guidance on how to perform immunostaining of the organoids. This protocol has been validated for mice and does not require fluorescence-activated cell purification.For complete details on the use and execution of this protocol, please refer to Gameiro et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (6)

Juliana Gutschow Gameiro

Jussi Virtanen

Liam Lee

James E. Schwob

Marco Aurélio Fornazieri

Brian Lin

Format Sitasi

APA MLA BibTeX

Gameiro, J.G., Virtanen, J., Lee, L., Schwob, J.E., Fornazieri, M.A., Lin, B. (2025). Protocol for culturing olfactory epithelium organoids supporting neuronal differentiation. https://doi.org/10.1016/j.xpro.2025.104211

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Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2025.104211
Akses: Open Access ✓