DOAJ Open Access 2025

Protocol for the generation of three-dimensional micropatterned neuroepithelial tissues using hPSCs, bioprinting, and matrix scaffolds

Kenneth Kin Lam Wong Negin Imani Farahani George Allen Abhimanyu Minhas Lisa M. Julian

Abstrak

Summary: Micropatterning technology that spatially guides the self-assembly of human pluripotent stem cells (hPSCs) into neural tissues offers enhanced fidelity to investigate early neurodevelopment and disease mechanisms. Here, we present a protocol to produce micropatterned and scaffolded neuroepithelial tissues (scNETs) from hPSCs, leveraging three-dimensional (3D) bioprinting to deposit extracellular matrix (ECM) droplets with defined geometries. We describe steps for bioprinting of ECM micropatterns, hPSC culture and seeding, and the subsequent neural induction process to form scNETs within 5 days.For complete details on the use and execution of this protocol, please refer to Imani Farahani et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (5)

Kenneth Kin Lam Wong

Negin Imani Farahani

George Allen

Abhimanyu Minhas

Lisa M. Julian

Format Sitasi

APA MLA BibTeX

Wong, K.K.L., Farahani, N.I., Allen, G., Minhas, A., Julian, L.M. (2025). Protocol for the generation of three-dimensional micropatterned neuroepithelial tissues using hPSCs, bioprinting, and matrix scaffolds. https://doi.org/10.1016/j.xpro.2025.104196

Akses Cepat

PDF tidak tersedia langsung

Cek di sumber asli →

Lihat di Sumber doi.org/10.1016/j.xpro.2025.104196

Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2025.104196
Akses: Open Access ✓