DOAJ Open Access 2025

Protocol for quantifying the thickness and puncture resistance properties of solitary bee cocoons using Osmia lignaria as a model

Oran Wasserman Mallory R. Wootton Spencer Fairbanks Brianne E. Bell Mary-Kate F. Williams +1 lainnya

Abstrak

Summary: Solitary bee cocoons serve as protective barriers against parasitoids, yet their puncture resistance properties remain understudied. Here, we present a protocol for measuring puncture resistance in Osmia lignaria cocoons using custom 3D-printed fixtures. We describe steps for determining bee sex, preparing cocoon segments, measuring segment thickness, testing puncture resistance using 27.5-gauge sharp needles on an MTS Synergie 100 mechanical testing instrument, and analyzing the data. The protocol generates quantitative data on puncture resistance properties and thickness measurements for comparative studies. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (6)

Oran Wasserman

Mallory R. Wootton

Spencer Fairbanks

Brianne E. Bell

Mary-Kate F. Williams

Justin A. Jones

Format Sitasi

APA MLA BibTeX

Wasserman, O., Wootton, M.R., Fairbanks, S., Bell, B.E., Williams, M.F., Jones, J.A. (2025). Protocol for quantifying the thickness and puncture resistance properties of solitary bee cocoons using Osmia lignaria as a model. https://doi.org/10.1016/j.xpro.2025.104191

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Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2025.104191
Akses: Open Access ✓