DOAJ Open Access 2025

Protocol for single-cell spatial transcriptomic profiling of cultured cells and engineered tissues without embedding or sectioning

Haylie R. Helms Luiz E. Bertassoni

Abstrak

Summary: Spatial transcriptomics is a powerful tool for investigating how cellular composition and spatial relationships influence cell behavior. Here, we present a protocol for spatial transcriptomic profiling of 2D engineered tissues and cell cultures that are not compatible with standard embedding and sectioning. We detail steps for sample generation, fixation, and staining to integrate with Visium HD spatial technology. This protocol provides a flexible solution for sample generation for spatial transcriptomic analysis. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (2)

Haylie R. Helms

Luiz E. Bertassoni

Format Sitasi

APA MLA BibTeX

Helms, H.R., Bertassoni, L.E. (2025). Protocol for single-cell spatial transcriptomic profiling of cultured cells and engineered tissues without embedding or sectioning. https://doi.org/10.1016/j.xpro.2025.104074

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Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2025.104074
Akses: Open Access ✓