DOAJ Open Access 2025

Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry

Coralie Berthoux Pablo E. Castillo Kaoutsar Nasrallah

Abstrak

Summary: Genetically encoded fluorescent sensors are powerful tools for tracking real-time neuromodulator dynamics. Here, we present a protocol to measure adenosine release using the G protein-coupled receptor activation-based adenosine (GRABAdo1.0m) sensor in the mouse hippocampus. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, ex vivo two-photon live imaging, and in vivo fiber photometry. We then detail histological procedures for experimental validation.For complete details on the use and execution of this protocol, please refer to Nasrallah et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (3)

Coralie Berthoux

Pablo E. Castillo

Kaoutsar Nasrallah

Format Sitasi

APA MLA BibTeX

Berthoux, C., Castillo, P.E., Nasrallah, K. (2025). Protocol to study adenosine release in the adult mouse hippocampus using a genetically encoded sensor, 2-photon live imaging, and fiber photometry. https://doi.org/10.1016/j.xpro.2025.103996

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Informasi Jurnal

Tahun Terbit: 2025
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2025.103996
Akses: Open Access ✓