DOAJ Open Access 2024

Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice

Thao Nguyen Toshiaki Yoshikawa Yusuke Ito Hitomi Kasuya Takahiro Nakashima +7 lainnya

Abstrak

Summary: Chimeric antigen receptor (CAR) T cell therapy often causes serious toxicities, such as cytokine release syndrome (CRS), mainly due to interleukin-6 (IL-6) secreted by monocyte lineage cells. Here, we describe a protocol to generate anti-CD19 CAR T cells and quantify human monocyte-derived IL-6 cocultured with CAR T cells and target tumor cells in vitro. We further describe a protocol to generate a humanized mouse model and evaluate CAR T cell-associated plasma IL-6 levels in vivo.For complete details on the use and execution of this protocol, please refer to Yoshikawa et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (12)

Thao Nguyen

Toshiaki Yoshikawa

Yusuke Ito

Hitomi Kasuya

Takahiro Nakashima

Sachiko Okamoto

Yasunori Amaishi

Haosong Zhang

Yang Li

Tetsuya Matsukawa

Satoshi Inoue

Yuki Kagoya

Format Sitasi

APA MLA BibTeX

Nguyen, T., Yoshikawa, T., Ito, Y., Kasuya, H., Nakashima, T., Okamoto, S. et al. (2024). Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice. https://doi.org/10.1016/j.xpro.2024.103423

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Informasi Jurnal

Tahun Terbit: 2024
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2024.103423
Akses: Open Access ✓