DOAJ Open Access 2024

Protocol to synthesize the auxin analog 5-Ph-IAA for conditional protein depletion in C. elegans using the AID2 system

Surojit Sural Juan Quintero Botero Oliver Hobert Makeda Tekle-Smith

Abstrak

Summary: The auxin-inducible degron (AID) system is a broadly used tool for spatiotemporal and reversible control of protein depletion in multiple experimental model systems. AID2 technology relies on a synthetic ligand, 5-phenyl-indole-3-acetic acid (5-Ph-IAA), for improved specificity and efficiency of protein degradation. Here, we provide a protocol for cost-effective 5-Ph-IAA synthesis utilizing the Suzuki coupling of 5-chloroindole and phenylboronic acid. We describe steps for evaluating the quality of lab-synthesized 5-Ph-IAA using a C. elegans AID2 tester strain. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (4)

Surojit Sural

Juan Quintero Botero

Oliver Hobert

Makeda Tekle-Smith

Format Sitasi

APA MLA BibTeX

Sural, S., Botero, J.Q., Hobert, O., Tekle-Smith, M. (2024). Protocol to synthesize the auxin analog 5-Ph-IAA for conditional protein depletion in C. elegans using the AID2 system. https://doi.org/10.1016/j.xpro.2024.102901

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Informasi Jurnal

Tahun Terbit: 2024
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2024.102901
Akses: Open Access ✓