DOAJ Open Access 2023

Protocol for altering connexin hemichannel function in primary chicken lens fiber cells using high-titer retroviral RCAS(A) infection

Yu Du Francisca M. Acosta Jianping Zhang Yuxin Tong Yumeng Quan +2 lainnya

Abstrak

Summary: Connexins (Cxs) play a crucial role in maintaining lens transparency. Here, we present a protocol for altering Cx hemichannel (HC) function in primary chicken lens fiber cells using high-titer retroviral replication competent avian sarcoma-leukosis virus long terminal repeat with splice acceptor (A) infection. We describe steps for incubating eggs, isolating lenses, culturing cells, preparing reagents, and infecting cells. We then detail cell treatment and detection of apoptosis and death. This protocol can assess protein kinase A, HC activity, and increased glutathione transport for protecting lens fiber cells against oxidative stress.For complete details on the use and execution of this protocol, please refer to Liu et al.,1 Riquelme et al.,2 Shi et al.,3 Jiang,4 and Rath et al.5 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (7)

Yu Du

Francisca M. Acosta

Jianping Zhang

Yuxin Tong

Yumeng Quan

Sumin Gu

Jean X. Jiang

Format Sitasi

APA MLA BibTeX

Du, Y., Acosta, F.M., Zhang, J., Tong, Y., Quan, Y., Gu, S. et al. (2023). Protocol for altering connexin hemichannel function in primary chicken lens fiber cells using high-titer retroviral RCAS(A) infection. https://doi.org/10.1016/j.xpro.2023.102564

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2023.102564
Akses: Open Access ✓