DOAJ Open Access 2023

Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope

Ziyuan Peng Yuling Jiao Ying Wang

Abstrak

Summary: Live imaging through confocal laser scanning microscopy enables the recording, analysis, and comparison of the dynamics of shapes and gene expression patterns of plant shoot apical meristems (SAMs) or primordia. Here, we provide a protocol to describe the preparation process of imaging Arabidopsis SAMs and primordia using a confocal microscope. We describe steps for dissection, visualization of meristems using dyes and fluorescent proteins, and gain 3D morphology of meristems. We then detail analysis of shoot meristems using time-lapse imaging.For complete details on the use and execution of this protocol, please refer to Peng et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (3)

Ziyuan Peng

Yuling Jiao

Ying Wang

Format Sitasi

APA MLA BibTeX

Peng, Z., Jiao, Y., Wang, Y. (2023). Live imaging of Arabidopsis shoot primordia via a confocal laser scanning microscope. https://doi.org/10.1016/j.xpro.2023.102217

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2023.102217
Akses: Open Access ✓