DOAJ Open Access 2023

In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells

Sanne van der Heijden Donovan Flumens Maarten Versteven Stefanie Peeters Hans De Reu +8 lainnya

Abstrak

Summary: Wilms’ tumor protein 1 (WT1) is a tumor-associated antigen overexpressed in various cancers. As a self-antigen, negative selection reduces the number of WT1-specific T cell receptors (TCRs). Here, we provide a protocol to generate WT137-45-specific TCRs using healthy human peripheral blood mononuclear cells. We describe the expansion of WT1-specific T cell clones by two consecutive in vitro stimulations with autologous WT137-45-pulsed dendritic cells and peripheral blood lymphocytes. We then detail the detection with human leukocyte antigen/WT137-45 tetramers. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Topik & Kata Kunci

Science (General)

Penulis (13)

Sanne van der Heijden

Donovan Flumens

Maarten Versteven

Stefanie Peeters

Hans De Reu

Diana Campillo-Davo

Yannick Willemen

Benson Ogunjimi

Viggo Van Tendeloo

Zwi N. Berneman

Sébastien Anguille

Evelien Smits

Eva Lion

Format Sitasi

APA MLA BibTeX

Heijden, S.v.d., Flumens, D., Versteven, M., Peeters, S., Reu, H.D., Campillo-Davo, D. et al. (2023). In vitro expansion of Wilms’ tumor protein 1 epitope-specific primary T cells from healthy human peripheral blood mononuclear cells. https://doi.org/10.1016/j.xpro.2023.102053

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Informasi Jurnal

Tahun Terbit: 2023
Sumber Database: DOAJ
DOI: 10.1016/j.xpro.2023.102053
Akses: Open Access ✓